Gpx4 Knockout cell line (MODE-K)

ENCN
location： Home > Products > KO Cell Lines > Gpx4 Knockout cell line (MODE-K)

Gpx4 Knockout cell line (MODE-K) Order

Gpx4 Knockout cell line (MODE-K)
Catalog#
YKO-M156
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Gpx4 Knockout cell line (MODE-K)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Gpx4 Knockout cell line（MDCK(NBL-2)）
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Gpx4
Gene ID
625249
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%MEM+10%FBS
Accession
CVCL_E0Z8(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript Gpx4-201)
Validation: PCR+Sanger Sequencing
Gpx4 Knockout cell line (MODE-K) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MDCK(NBL-2)		 STR Info (Cell bank)
Cell Line:NALM-6
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 811811
D16S539 10111011
VWA 15161516
TH01 8989
AMEL XYXY
TPOX 810810
CSF1PO 13131313
D12S391 2021
FGA 2223
D2S1338 1724
D21S11 2729
D18S51 1215
D8S1179 1213
D3S1358 1617
D6S1043 1113
PENTAE 1111
D19S433 1315
PENTAD 814
D1S1656 111217.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Gpx4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDCK(NBL-2) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Gpx4 gene knockout Gpx4 Knockout cell line（MDCK(NBL-2)） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Gpx4 Knockout cell line (MODE-K)
Gpx4 Knockout cell line (MODE-K)
Catalog#
YKO-M156
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GPX4 Knockout cell line (HCT 116)

    GPX4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: GPX4
    Gene ID: 2879
    Size: 1*10^6
    Catalog#: YKO-KP248
    YKO-KP248(HCT 116)
  • Gpx4 Knockout cell line(RAW 264.7)

    Gpx4 Knockout cell line(RAW 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Gpx4
    Gene ID: 625249
    Size: 1*10^6
    Catalog#: YKO-M109
    YKO-M109(RAW 264.7)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • GPX4 Knockout cell line (HCT 116)

    GPX4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GPX4
    Gene ID: 2879
    Size: 1*10^6
    Catalog#: YKO-KP248
      inquiry   More details
  • Gpx4 Knockout cell line(RAW 264.7)

    Gpx4 Knockout cell line(RAW 264.7)

    Organism: Mouse
    Gene Name: Gpx4
    Gene ID: 625249
    Size: 1*10^6
    Catalog#: YKO-M109
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now